| 1 |  Shirin van Anrooij (P-B) | | 3:39:32 |
| 2 |  Elisa Balsamo (ITA) | Trek - Segafredo | 0:23 |
| 3 | Vittoria Guazzini (ITA) | Fdj - Suez | mt |
| 4 |  Arlenis Sierra Cañadilla (CUB) | Movistar Team | mt |
| 5 | Soraya Paladin (ITA) | Canyon - Sram Racing | mt |
| 6 | Silvia Persico (ITA) | Uae Team Adq | mt |
| 7 | Elise Uijen (P-B) | Team Dsm | mt |
| 8 |  Justine Ghekiere (BEL) | AG Insurance - Soudal Quick-Step | mt |
| 9 |  Claire Steels (G-B) | Team Jayco Alula | mt |
| 10 |  Margarita Victoria García Cañellas (ESP) | Liv Racing Teqfind | mt |
| 11 | Yara Kastelijn (P-B) | Fenix-Deceuninck | mt |
| 12 |  Juliette Labous (FRA) | Team Dsm | 0:26 |
| 13 | Marta Cavalli (ITA) | Fdj - Suez | mt |
| 14 |  Ricarda Bauernfeind (ALL) | Canyon - Sram Racing | mt |
| 15 |  Katarzyna Niewiadoma (POL) | Canyon//sram Generation | mt |
| 16 |  Amanda Spratt (AUS) | Trek - Segafredo | 0:30 |
| 17 | Gaia Realini (ITA) | Trek - Segafredo | mt |
| 18 | Lorena Wiebes (P-B) | Team SD Worx | 1:30 |
| 19 | Grace Brown (AUS) | Fdj - Suez | mt |
| 20 | Marianne Vos (P-B) | Team Jumbo - Visma | 1:31 |
| 21 | Loes Adegeest (P-B) | Fdj - Suez | mt |
| 22 | Alessia Vigilia (ITA) | | mt |
| 23 | Karlijn Swinkels (P-B) | Team Jumbo - Visma | mt |
| 24 |  Elise Chabbey (SUI) | Canyon - Sram Racing | mt |
| 25 | Ane Santesteban González (ESP) | Groupama - Fdj | mt |
| 26 | Francesca Barale (ITA) | Team Dsm | mt |
| 27 | Debora Silvestri (ITA) | | mt |
| 28 | Mareille Meijering (P-B) | | mt |
| 29 | Esmée Peperkamp (P-B) | Team Dsm | mt |
| 30 |  Paula Andrea Patiño Bedoya (COL) | Movistar Team | mt |
| 31 | Pauliena Rooijakkers (P-B) | Canyon - Sram Racing | mt |
| 32 | Erica Magnaldi (ITA) | Uae Team Adq | mt |
| 33 |  Caroline Andersson (SUE) | Liv Racing Teqfind | mt |
| 34 |  Alena Amialyusik (BLR) | Uae Team Adq | 1:36 |
| 35 | Riejanne Markus (P-B) | Team Jumbo - Visma | 1:39 |
| 36 | Anna Shackley (G-B) | Team SD Worx | 1:45 |
| 37 |  Niamh Fisher-Black (NZL) | Team SD Worx | 3:03 |
| 38 | Sina Frei (SUI) | Team SD Worx | mt |
| 39 | Amber Kraak (P-B) | Team Jumbo - Visma | mt |
| 40 | Évita Muzic (FRA) | Fdj - Suez | mt |
| 41 |  Coryn Labecki (E-U) | Team Jumbo - Visma | 5:36 |
| 42 | Alice Maria Arzuffi (ITA) | Ceratizit - Wnt | mt |
| 43 | Danielle De Francesco (AUS) | | mt |
| 44 | Nadia Quagliotto (ITA) | Laboral Kutxa - Fundación Euskadi | mt |
| 45 | Mischa Bredewold (P-B) | Team SD Worx | mt |
| 46 | Ruby Roseman-Gannon (AUS) | Groupama - Fdj | mt |
| 47 | Kimberly Cadzow (NZL) | Team Jumbo - Visma | mt |
| 48 |  Anna Kiesenhofer (AUT) | Team Jayco Alula | mt |
| 49 | Eleonora Ciabocco (ITA) | Team Dsm | 5:40 |
| 50 | Marta Lach (POL) | Ceratizit - Wnt | 8:46 |
| 51 | Quinty Ton (P-B) | Liv Racing Teqfind | mt |
| 52 | Silvia Zanardi (ITA) | Be Pink | mt |
| 53 | Elena Pirrone (ITA) | Team Jayco Alula | mt |
| 54 | Tiffany Cromwell (AUS) | Canyon - Sram Racing | mt |
| 55 | Sabrina Stultiens (P-B) | Liv Racing Teqfind | mt |
| 56 | Ariana Gilabert Vilaplana (ESP) | Laboral Kutxa - Fundación Euskadi | mt |
| 57 | Marta Jaskulska (POL) | Liv Racing Teqfind | mt |
| 58 | Eneritz Vadillo Crespo (ESP) | Laboral Kutxa - Fundación Euskadi | mt |
| 59 | Mireia Benito Pellicer (ESP) | AG Insurance - Soudal Quick-Step | mt |
| 60 | Lone Meertens (BEL) | AG Insurance - Soudal Quick-Step | mt |
| 61 | Sara Martín Martín (ESP) | Movistar Team | mt |
| 62 |  Stine Anderson Borgli (NOR) | Fdj - Suez | mt |
| 63 | Heidi Franz (E-U) | | mt |
| 64 | Greta Marturano (ITA) | Fenix-Deceuninck | mt |
| 65 |  Alena Ivanchenko (RUS) | Uae Team Adq | mt |
| 66 | Beatrice Rossato (ITA) | Isolmant - Premac - Vittoria | mt |
| 67 | Brodie Chapman (AUS) | Trek - Segafredo | mt |
| 68 | Gaia Segato (ITA) | | mt |
| 69 |  Urska Zigart (SLO) | Groupama - Fdj | mt |
| 70 | Petra Stiasny (SUI) | Fenix-Deceuninck | mt |
| 71 | Giorgia Vettorello (ITA) | Be Pink | mt |
| 72 | Sofia Bertizzolo (ITA) | Uae Team Adq | 8:57 |
| DNF | Mikayla Harvey (NZL) | Uae Team Adq | - |
| DNF |  Rasa Rumsaite (LTU) | Aromitalia - Basso Bikes - Vaiano | - |
| DNF | Katrine Aalerud (NOR) | Movistar Team | - |
| DNF | Sara Casasola (ITA) | Born to Win G20 Ambedo | - |
| DNF | Tamara Dronova-Balabolina (RUS) | Team Jayco Alula | - |
| DNF | Rachele Bonzanini (ITA) | Born to Win G20 Ambedo | - |
| DNF |  Nikola Nosková (RTC) | | - |
| DNF | Eva Maria Gatscher (ITA) | | - |
| DNF | Iurani Blanco Calbet (ESP) | Laboral Kutxa - Fundación Euskadi | - |
| DNF | Elisa Lugli (ITA) | | - |
| DNF | Jennifer Tatiana Ducuara Acevedo (COL) | Massi - Tactic | - |
| DNF | Rebecca Rigamonti (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | - |
| DNF | Lara Scarselli (ITA) | Aromitalia - Basso Bikes - Vaiano | - |
| DNF | Sylvie Truc (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | - |
| DNF | Ingvild Gåskjenn (NOR) | Groupama - Fdj | - |
| DNF | Valentina Basilico (ITA) | Be Pink | - |
| DNF | Julie Van De Velde (BEL) | Fenix-Deceuninck | - |
| DNF |  Vanessa Santeliz Bautista (VEN) | Born to Win G20 Ambedo | - |
| DNF | Arianna Fidanza (ITA) | Ceratizit - Wnt | - |
| DNF | Noemi Lucrezia Eremita (ITA) | Isolmant - Premac - Vittoria | - |
| DNF | Anya Louw (AUS) | AG Insurance - Soudal Quick-Step | - |
| DNF | Angela Oro (ITA) | | - |
| DNF |  Petra Zsankó (HON) | Massi - Tactic | - |
| DNF | Alice Palazzi (ITA) | | - |
| DNF | Irene Affolati (ITA) | Aromitalia - Basso Bikes - Vaiano | - |
| DNF | Benedetta Della Corte (ITA) | | - |
| DNF | Léa Curinier (FRA) | Team Dsm | - |
| DNF | Prisca Savi (ITA) | Be Pink | - |
| DNF | Lourdes Oyarbide Jimenez (ESP) | Movistar Team | - |
| DNF | Giorgia Serena (ITA) | Born to Win G20 Ambedo | - |
| DNF | Silvia Magri (ITA) | Team Jayco Alula | - |
| DNF | Asia Zontone (ITA) | Isolmant - Premac - Vittoria | - |
| DNF | Marta Romance I Oms (ESP) | | - |
| DNF | Martina Sanfilippo (ITA) | | - |
| DNF | Laura Rodriguez Cordero (ESP) | | - |
| DNF | Giorgia Bariani (ITA) | | - |
| DNF |  Miryan Maritza Nuñez Padilla (EQU) | Massi - Tactic | - |
| DNF | Vittoria Ruffilli (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | - |
| DNF | Fanny Bonini (ITA) | | - |
| DNF | Alexandra Manly (AUS) | Groupama - Fdj | - |
| DNF | Letizia Brufani (ITA) | Be Pink | - |
| DNF | Sophie Wright (G-B) | Fenix-Deceuninck | - |
| DNF | Elisa Taiti (ITA) | Born to Win G20 Ambedo | - |
| DNF | Lana Eberle (ALL) | Ceratizit - Wnt | - |
| DNF | Ainara Albert Bosch (ESP) | Isolmant - Premac - Vittoria | - |
| DNF | Gaia Masetti (ITA) | AG Insurance - Soudal Quick-Step | - |
| DNF | Alice Capasso (ITA) | | - |
| DNF | Cécile Lejeune (FRA) | Massi - Tactic | - |
| DNF | Cristina Tonetti (ITA) | | - |
| DNF | Milena Del Sarto (ITA) | Aromitalia - Basso Bikes - Vaiano | - |
| DNF | Gemma Sernissi (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | - |
| DNF | Katia Ragusa (ITA) | Liv Racing Teqfind | - |
| DNF | Andrea Casagranda (ITA) | Be Pink | - |
| DNF | Marthe Truyen (BEL) | Fenix-Deceuninck | - |
| DNF | Sofia Barbieri (ITA) | Born to Win G20 Ambedo | - |
| DNF |  Thi That Nguyen (VIE) | Team Jayco Alula | - |
| DNF | Carmela Cipriani (ITA) | Isolmant - Premac - Vittoria | - |
| DNF | Marthe Goossens (BEL) | AG Insurance - Soudal Quick-Step | - |
| DNF | Monica Castagna (ITA) | | - |
| DNF | Aurela Nerlo (POL) | Massi - Tactic | - |
| DNF | Iris Monticolo (ITA) | | - |
| DNF | Rasa Leleivyte (LTU) | Aromitalia - Basso Bikes - Vaiano | - |
| DNF | Chiara Sacchi (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | - |
| DNS | Kristen Faulkner (E-U) | Groupama - Fdj | - |
