| 1 |  Elisa Longo Borghini (ITA) | Lidl - Trek | 2:53:20 |
| 2 |  Évita Muzic (FRA) | Fdj - Suez | 0:09 |
| 3 | Juliette Labous (FRA) | Team Dsm-Firmenich Post NL | mt |
| 4 |  Urska Zigart (SLO) | Liv-Alula-Jayco | 0:16 |
| 5 |  Mareille Meijering (P-B) | Movistar Team | 0:24 |
| 6 | Nienke Vinke (P-B) | Team Dsm-Firmenich Post NL | 0:36 |
| 7 | Monica Trinca Colonel (ITA) | | mt |
| 8 |  Katrine Aalerud (NOR) | Uno-X Mobility | mt |
| 9 | Maëva Squiban (FRA) | Arkéa - B&b Hotels | 0:41 |
| 10 | Cédrine Kerbaol (FRA) | Ceratizit - Wnt | mt |
| 11 |  Cecilie Uttrup Ludwig (DAN) | Fdj - Suez | mt |
| 12 |  Brodie Chapman (AUS) | Lidl - Trek | mt |
| 13 | Erica Magnaldi (ITA) | Uae Team Adq | 0:49 |
| 14 |  Margarita Victoria García Cañellas (ESP) | Liv-Alula-Jayco | 1:09 |
| 15 | Ingvild Gåskjenn (NOR) | Liv-Alula-Jayco | 1:20 |
| 16 | Loes Adegeest (P-B) | Fdj - Suez | 1:22 |
| 17 |  Olha Kulynych (UKR) | | 1:24 |
| 18 | Mie Bjørndal Ottestad (NOR) | Uno-X Mobility | 1:37 |
| 19 | Eleonora Camilla Gasparrini (ITA) | Uae Team Adq | 1:48 |
| 20 | Gaia Realini (ITA) | Lidl - Trek | mt |
| 21 | Julie Bego (FRA) | Cofidis Women Team | 1:53 |
| 22 |  Paula Andrea Patiño Bedoya (COL) | Movistar Team | 2:01 |
| 23 | Eleonora Ciabocco (ITA) | Team Dsm-Firmenich Post NL | 2:06 |
| 24 |  Nina Berton (LUX) | Ceratizit - Wnt | 2:08 |
| 25 | Barbara Malcotti (ITA) | Human Powered Health | 2:17 |
| 26 | Iurani Blanco Calbet (ESP) | Laboral Kutxa - Fundación Euskadi | 2:35 |
| 27 | Usoa Ostolaza Zabala (ESP) | Laboral Kutxa - Fundación Euskadi | mt |
| 28 |  Flora Perkins (G-B) | | mt |
| 29 | Yuliia Biriukova (UKR) | Human Powered Health | mt |
| 30 | Debora Silvestri (ITA) | Laboral Kutxa - Fundación Euskadi | mt |
| 31 | Edseth Marte Berg (NOR) | Uno-X Mobility | mt |
| 32 | Juliana Londoño (COL) | | 2:44 |
| 33 |  Caroline Andersson (SUE) | Liv-Alula-Jayco | mt |
| 34 | Sofia Arici (ITA) | | 2:47 |
| 35 | Laura Asencio (FRA) | Ceratizit - Wnt | 2:51 |
| 36 |  Rasa Leleivyte (LTU) | Aromitalia 3T Vaiano | mt |
| 37 | Francesca Barale (ITA) | Team Dsm-Firmenich Post NL | 3:08 |
| 38 | Ema Comte (FRA) | | mt |
| 39 | Titia Ryo (FRA) | Arkéa - B&b Hotels | 3:10 |
| 40 | Sofia Bertizzolo (ITA) | Uae Team Adq | 3:13 |
| 41 | Beatrice Rossato (ITA) | | 3:19 |
| 42 |  Carina Schrempf (AUT) | Fenix-Deceuninck | 3:25 |
| 43 | Vittoria Ruffilli (ITA) | A.S.D. K2 Women Team | mt |
| 44 | Elisa Valtulini (ITA) | | 3:27 |
| 45 | Clémence Latimier (FRA) | | 3:38 |
| 46 | Katia Ragusa (ITA) | Human Powered Health | 3:45 |
| 47 | Giulia Giuliani (ITA) | A.S.D. K2 Women Team | mt |
| 48 | Chiara Reghini (ITA) | Top Girls Fassa Bortolo | 3:49 |
| 49 |  Ruth Edwards (Winder) (E-U) | Human Powered Health | 3:59 |
| 50 | Noemi Lucrezia Eremita (ITA) | A.S.D. K2 Women Team | 4:01 |
| 51 |  Hannah Ludwig (ALL) | Cofidis Women Team | 4:07 |
| 52 | Elinor Barker (G-B) | Uno-X Mobility | 4:15 |
| 53 | Irene Affolati (ITA) | Aromitalia 3T Vaiano | mt |
| 54 | Nika Bobnar (SLO) | | 4:36 |
| 55 | Monica Castagna (ITA) | Top Girls Fassa Bortolo | 4:50 |
| 56 | Giada Borghesi (ITA) | | 4:53 |
| 57 | Andrea Casagranda (ITA) | | 4:57 |
| 58 | Serena Brillante Romeo (ITA) | Aromitalia 3T Vaiano | 5:03 |
| 59 | Joscelin Lowden (G-B) | Uno-X Mobility | mt |
| 60 | Josie Nelson (G-B) | Team Dsm-Firmenich Post NL | 5:20 |
| 61 | Valeria Curnis (ITA) | | mt |
| 62 | Claire Steels (G-B) | Movistar Team | mt |
| 63 | Sara Piffer (ITA) | | mt |
| 64 | Sara Pepoli (ITA) | | 5:34 |
| 65 | Elisa De Vallier (ITA) | Top Girls Fassa Bortolo | mt |
| 66 | Sara Martín Martín (ESP) | Movistar Team | 5:37 |
| 67 | Alice Papo (ITA) | A.S.D. K2 Women Team | 5:48 |
| 68 |  Yulduz Hashimi (AFG) | | 5:51 |
| 69 | Iris Monticolo (ITA) | Top Girls Fassa Bortolo | 6:00 |
| 70 |  Hanna Tserah (BLR) | | 6:02 |
| 71 | Angela Oro (ITA) | | mt |
| 72 |  Dominika Wlodarczyk (POL) | Uae Team Adq | 6:06 |
| 73 |  Shaknoza Abdullaeva (UZB) | | 6:18 |
| 74 | Marta Pavesi (ITA) | Top Girls Fassa Bortolo | 6:25 |
| 75 | Marie Le Net (FRA) | Fdj - Suez | 6:32 |
| 76 | Michela De Grandis (ITA) | | 6:38 |
| 77 | Maurène Trégouët (FRA) | Arkéa - B&b Hotels | mt |
| 78 | Laura Tomasi (ITA) | Laboral Kutxa - Fundación Euskadi | mt |
| 79 | Vittoria Guazzini (ITA) | Fdj - Suez | mt |
| 80 |  Argiro Milaki (GRE) | | mt |
| 81 | Lara Crestanello (ITA) | | mt |
| 82 | Cat Ferguson (G-B) | | mt |
| 83 | Cristina Tonetti (ITA) | Laboral Kutxa - Fundación Euskadi | mt |
| 84 | Matilde Vitillo (ITA) | Liv Alula Jayco's Continental Team | mt |
| 85 | Nadia Quagliotto (ITA) | Laboral Kutxa - Fundación Euskadi | mt |
| 86 | Irene Cagnazzo (ITA) | | mt |
| 87 | Virginia Bortoli (ITA) | Top Girls Fassa Bortolo | mt |
| 88 | Silvia Zanardi (ITA) | Human Powered Health | mt |
| 89 | Giulia Vallotto (ITA) | | mt |
| 90 | Romina Costantini (ITA) | | mt |
| 91 | Dziyana Lebedz (BLR) | | 6:52 |
| 92 | Flavie Boulais (FRA) | Cofidis Women Team | 7:18 |
| 93 | Linda Ferrari (ITA) | | 7:20 |
| 94 | Lana Eberle (ALL) | Ceratizit - Wnt | mt |
| 95 | Anastasia Carbonari (ITA) | Uae Team Adq | 7:41 |
| 96 | Sara Pellegrini (ITA) | A.S.D. K2 Women Team | 8:36 |
| 97 | Valentina Zanzi (ITA) | Aromitalia 3T Vaiano | 9:07 |
| 98 | Becky Storrie (G-B) | Team Dsm-Firmenich Post NL | 9:24 |
| 99 |  Yelizaveta Sklyarova (KAZ) | | 9:40 |
| DNF | Nina Buijsman (P-B) | Fdj - Suez | - |
| DNF | Pauliena Rooijakkers (P-B) | Fenix-Deceuninck | - |
| DNF | Greta Marturano (ITA) | | - |
| DNF |  Petra Stiasny (SUI) | Fenix-Deceuninck | - |
| DNF | Felicity Wilson-Haffenden (AUS) | Lidl - Trek | - |
| DNF | Elizabeth Deignan-Armitstead (G-B) | Lidl - Trek | - |
| DNF | Lauretta Hanson (AUS) | Lidl - Trek | - |
| DNF | Anna Trevisi (ITA) | | - |
| DNF | Alena Amialyusik (BLR) | Uae Team Adq | - |
| DNF | Viktoriia Melnychuk (UKR) | | - |
| DNF |  Lotte Claes (BEL) | Arkéa - B&b Hotels | - |
| DNF | Océane Mahé (FRA) | Arkéa - B&b Hotels | - |
| DNF | Ainhoa Moreno Ossowsky (ESP) | Aromitalia 3T Vaiano | - |
| DNF | Ursa Pintar (SLO) | | - |
| DNF | Spela Colnar (SLO) | | - |
| DNF | Spela Kern (SLO) | Cofidis Women Team | - |
| DNF |  Nikola Nosková (RTC) | Cofidis Women Team | - |
| DNF | Emanuela Zanetti (ITA) | | - |
| DNF | Sonia Rossetti (ITA) | | - |
| DNF | Ilaria Prevedello (ITA) | | - |
| DNF | Giulia Miotto (ITA) | | - |
